Measuring Microfibrillar Angles Using Light Microscopy
Keywords:
Microfibril angle, microscopy, cell wallsAbstract
A rapid, reliable method of enhancing microfibrillar angles for viewing in wood cell walls, a modification of I. W. Bailey's technique, is reviewed. Dried microtomed sections are treated with an iodinepotassium iodide solution and nitric acid to produce crystals in cell walls of softwood tracheids or hardwood fibers. The crystals are aligned with the predominant microfibrillar angle and may be easily viewed and measured.References
Bailey, I. W., and M. R. Vestal. 1937. The orientation of cellulose in the secondary wall of tracheary cells. J. Arnold Arboretum 18(3):185-195.nKobayashi, Y. 1952. A simple method for the preparation of samples for measurement of inclination of micelle in the wood cell wall. J. Jap. For. Society 34(12).n
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