Utilization of Soft-rot Cavity Orientation for the Determination of Microfibril Angle. Part I
Keywords:
Cellulose, fibers, iodine crystal deposition, microfibril angle, S2 angle, soft-rot cavities, tracheids, X-ray diffractionAbstract
These studies utilize the decay cavities formed by the soft-rot fungus, Phialocephala dimorphospora, to determine the orientation of the cellulose fibrils in the cell wall. In this study, the microfibril angle was determined utilizing three methods: X-ray diffraction (T method), iodine staining, and orientation of the soft-rot cavities. The results demonstrate good agreement between the three techniques and verify that the decay cavities are formed in a direction parallel to the cellulose microfibrils and therefore can be used to determine the orientation of the cellulose microfibrils. One advantage of the soft-rot method over the X-ray method is the ability to measure angles of any size, including those of juvenile wood.References
Anagnost, S. E. 1998. Light microscopic diagnosis of wood decay. Int. Assoc. Wood Anat. J. 19(2): 141-167.nAnagnost, S. E., J. J. Worral., and C. J. K. Wang. 1994. Diffuse cavity formation in soft rot of pine. Wood Sci. Technol. 28:199-208.nAnagnost, S. E., R. E. Mark, and R. B. Hanna. 1999. Utilization of soft rot cavity orientation for the determination of microfibril angle, Part 2. J. Pulp Paper Sci. Submitted.nBailey, I. W., and M. R. Vestal. 1937. The significance of certain wood-destroying fungi in the study of enzymatic hydrolysis of cellulose. J. Arnold Arboretum 18: 196-205.nCourtois, H. 1963. Micromorphological symptoms of wood deterioration by soft rot fungi. Holzforsch. Holz-verwert. 15(5):89-101.nCrossley, A., and J. F. Levy. 1977. Proboscis hyphac in soft rot cavity formation. J. Inst. Wood Sci. 7:30-33.nEl-Hosseiny, F., and D. H. Page. 1973. The measurement of fibril angle of wood fibers using polarized light. Wood Fiber 5(3):208-214.nHale, M. D. C., and R. A. Eaton. 1983. Soft rot decay of wood: The infection and cavity-forming processes of Phialophora hoffmannii (Van Beyma) Schol-Schwarz. Pages 54-63 in T. A. Oxley and S. Barry, eds. Biodeterioration 5. Wiley-Interscience, Chichester, UK.nHale, M. D. C., and R. A. Eaton. 1985. Oscillatory growth of fungal hyphac in wood cell walls. Trans. Br. Mycol. Soc. 84(2): 277-288.nHuang, C-L., N. P. Kutscha, G. P. Leaf, and R. A. Megraw. 1998. Comparison of microfibril angle measurement techniques. Pages 177-205 in B. Butterfield, ed. Microfibril angle in wood. University of Canterbury, Christchurch, NZ.nJutte, S. M., and A. B. Wardrop. 1970. Morphological factors relating to the degradation of wood fibers by cellulasc preparations. Acta Bot. Neerl. 19(6):906-917.nKretschmann, D., H. A. Alden, and S. Verrill. 1998. Variations of microfibril angle in loblolly pine: comparison of iodine crystallization and x-ray diffraction techniques. Pages 157-176 in B. Butterfield, ed. Micro-fibril angle in wood. University of Canterbury, Christ-church, NZ.nLevi, M. P., and R. D. Preston. 1965. A chemical and microscopic examination of the action of the soft-rot fungus Chaetomium globosum on beechwood (Fagus sylv.). Holzforschung 19:183-190.nMeylan, B. A. 1967. Measurement of microfibril angle by x-ray diffraction. Forest Prod. J. 17(5):51-58.nNilsson, T. 1974. Microscopic studies on the degradation of cellophane and various cellulosic fibers by wood attacking microfungi. Studia Forestalia Suecica Nr. 117, Stockholm, Sweden. 32 pp.nPage, D. H. 1969. A method for determining fibrillar angle in wood tracheids. J. Roy. Micros. Soc. 90:139-143.nSenft, J. F., and B. A. Bendtsen. 1985. Measuring microfibrillar angles using light microscopy. Wood Fiber Sci. 17(4):564-567.nShupe, T., E. T. Choong, D. D. Stokke, and M. D. Gibson. 1996. Variation in cell dimensions and fibril angle for two fertilized even-aged loblolly pine plantations. Wood Fiber Sci. 28(2):268-275.nWardrop, A. B., and S. M. Jutte. 1968. The enzymatic degradation of cellulose from Valonia ventricosa. Wood Sci. Technol. 2:105-114.nWorrall, J. J., S. E. Anagnost, and C. J. K. Wang. 1991. Conditions for soft rot of wood. Can. J. Microbiol. 37:869-874.n
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