Influence of Carbon Source on Cellulase Activity of White-rot and Brown-rot Fungi


  • Terry L. Highley


<i>Polyporus versicolor</i>, <i>Ganoderma applanatum</i>, <i>Peniophora "G"</i>, <i>Poria monticola</i>, <i>Lentinus lepideus</i>, <i>Lenzites trabea</i>, enzyme, decay


Three white-rot fungi, Polyporus versicolor, Ganoderma applanatum, and Peniophora "G," produce an adaptive cellulase complex that can degrade both soluble cellulose (Cx) and microcrystalline cellulose (C1), a highly ordered form of cellulose. Production of Cx and C1 by the white-rot fungi was repressed by simple sugars. Cellulase preparations from three brown-rot fungi, Poria monticola, Lentinus lepideus, and Lenzites trabea, exhibited only Cx activity; microcrystalline cellulose was not significantly degraded. Contrary to the cellulase (Cx) of the white-rot fungi, that of the brown-rot fungi apparently is constitutive, since activity was abundant in cultures with simple sugars or with non-cellulosic polysac-charides as the sole source of carbon. This work disclosed no differences between the cellulase-inducing effects of hardwoods versus those of softwoods that might help explain the preference of white rotters for hardwoods and brown rotters for softwoods.


Bailey, P. J., W. Liese, R. Roesch, G. Keilich, and E. G. Afting. 1969. Cellulase (B-1, 4-Clucan, 4-Glucanohydrolase) from wood-degrading fungus Polyporus schweinitzii Fr. I. Purification. Biochim. Biophys. Acta 185:381-391.nBarash, I., and L. Klein. 1969. The surface localization of polygalacturonase in spores of Geotrichum candidum.Phytopathology 59:319-324.nCowling, E. B. 1961. Comparative biochemistry of the decay of sweetgum sapwood by white-rot and brown-rot fungi. USDA For. Serv. Tech. Bull. 1258. 79 pp.nCowling, E. B., and W. Brown. 1969. Structural features of cellulosic materials in relation to enzymatic hydrolysis. In G. J. Hajny and E. T. Reese, eds., Cellulases and their applications. Adv. Chem. Ser. 95:152-187.nJensen, K. F. 1971. Cellulolytic enzymes of Stereum gausapatum.Phytopathology 61:134-138.nJohansson, M. 1966. A comparison between the cellulolytic activity of white and brown rot fungi. I. The activity on insoluble cellulose. Physiol. Plant. 19:709-722.nKeilich, G., P. J. Bailey, E. G. Afting, and W. Liese. 1969. Cellulase (B-1, 4-Glucan, 4-Glucanohydrolase) from the wood-degrading fungus Polyporus schweinitizii Fr. II. Characterization. Biochini. Biophys. Acta 185:392-401.nKing, N. J. 1968. Degradation of holocellulose by an enzyme preparation from a wood-degrading fungus. Nature 218(5147):1173-1174.nKoenigs, J. W. 1972. Effects of hydrogen peroxide on cellulose and on its susceptibility to cellulose. Mater. Org. 7(2):133-147.nLowry, O. H., N. J. Rosebrough, A. L. Farr, and R. J. Randall. 1951. Protein measurements with the folin phenol reagent. J. Biol. Chem. 193:265-275.nLumsden, R. D. 1969. Sclerotinia sclerotinum infection of bean and the production of cellulase. Phytopathology 59:653-657.nMandels, M., and J. Weber. 1969. The production of cellulases. In G. J. Hajny and E. T. Reese, eds., Cellulases and their applications. Adv. Chem. Ser. 95:391-414.nMarsii, P. B., and E. T. Reese. 1963. Measurement of cellulase. Page 91 in E. T. Reese, ed., Advances in enzymic hydrolysis of cellulose and related materials. Pergamon, Oxford.nNelson, N. 1944. A photometric adaptation of the Somogyi method for the determination of glucose. J. Biol. Chem. 153:375-380.nReese, E. T., and H. S. Levinson. 1952. A comparative study of the breakdown of cellulose by microorganisms. Physiol. Plant. 5:345-366.nWalch, H., and H. Kühlwein. 1968. Zur Kenntnis der cellulolytischen Aktivatät in der Gattung Ganoderma (Lackporlinge). Arch. Mikrobiol. 61:373-380.n






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