Biological durability of preservative treated cross-laminated timber (CLT) made with southern pine lumber

Authors

  • Bradia Henfield Arxada
  • Tamara Suely Filgueira Amorim França Mississippi State University
  • Frederico Franca Mississippi State University
  • Franklin Quin Jr Mississippi State University
  • Katie Ohno USDA Forest Products Laboratory
  • Rachel Arango USDA Forest Products Laboratory

Abstract

Cross-laminated timber (CLT) is an engineered wood product that is being increasingly utilized in structural applications. However, its susceptibility to fungal decay and termite attack raises concerns about long-term durability, particularly in high-moisture and insect-prone environments. This study evaluates the biological durability of CLT fabricated with southern pine and treated post-layup with two copper-based preservatives: copper azole (CA-C) and micronized copper azole (MCA) under laboratory conditions. Fungal decay resistance was assessed using brown-rot (Serpula lacrymans) and white-rot (Irpex lacteus) fungi, while termite resistance was evaluated through exposure to Reticulitermes spp. Results showed that preservative treatments significantly reduced fungal decay and termite damage compared to untreated controls, although treatment effectiveness varied with CLT configuration (3-ply or 5-ply). While both preservatives effectively mitigated decay in 3-ply panels, treated 5-ply panels exhibited comparable mass loss to untreated counterparts. Termite resistance tests demonstrated that MCA and CA-C treated CLT samples exhibited significantly lower mass loss and higher termite mortality compared to the untreated controls, with both preservatives providing comparable protection.

References

Akgul A, Akgul A (2018) Mycoremediation of copper: Exploring the metal tolerance of brown rot fungi. Biores 13(3):7155–7171. https://bioresources.cnr.ncsu.edu/resources/mycoremediation-of-copper-exploring-the-metal-tolerance-of-brown-rot-fungi/

Arango RA, Green F, Hintz K, Lebow PK, Miller RB (2006) Natural durability of tropical and native woods against termite damage by Reticulitermes flavipes (Kollar). Int Biodeter Biodegr 57(3):146–150. https://doi.org/10.1016/j.ibiod.2006.01.007

Archer K, Lebow S (2006) Wood preservation. In Primary Wood Processing: Principles and Practice. Springer, Dordrecht, pp. 297–338. https://doi.org/10.1007/1-4020-4393-7_9

AWPA (2023a) P61-16 Standard for Micronized Copper Azole (MCA), American Wood Protection Association, Birmingham, AL.

AWPA (2023b) U1-23 Use Category System: User Specification for Treated Wood, American Wood Protection Association, Birmingham, AL.

AWPA (2023c) E10-16 Laboratory method for evaluating the decay resistance of wood-based materials against pure basidiomycete cultures: soil/block test. American Wood Protection Association, Birmingham, AL.

AWPA (2023d) E1-23 Laboratory methods for evaluating the termite resistance of wood-based materials: choice and no-choice tests. American Wood Protection Association, Birmingham, AL.

Ayanleye S, Quin F, Zhang X, Lim H, Shmulsky R (2023) Preservatives penetration and retention in post-treated cross-laminated timber panels with different layup and thickness. J Build Eng 67:106009. https://doi.org/10.1016/j.jobe.2023.106009

Brischke C, Alfredsen G (2020) Wood-water relationships and their role for wood susceptibility to fungal decay. Appl Microbiol and Biotechnol 104:3781–3795. https://doi.org/10.1007/s00253-020-10479-1

Calovi M, Zanardi A, Rossi S (2024) Recent advances in bio-based wood protective systems: A comprehensive review. Appl Sci 14(2):736. 10.3390/app14020736

Capelari M, Zadrazil F (1997) Lignin degradation and in vitro digestibility of wheat straw treated with Brazilian tropical species of white rot fungi. Folia Microbiol 42:481–487. https://doi.org/10.1007/BF02826558

Civardi C, Schwarze FWMR, Wick P (2015) Micronized copper wood preservatives: an efficiency and potential health risk assessment for copper-based nanoparticles. Environ Pollut 200:126–132. https://doi.org/10.1016/j.envpol.2015.02.018

Clausen CA, Kartal SN, Arango R, Green III F (2011) The role of particle size of particulate nano-zinc oxide wood preservatives on termite mortality and leach resistance. Nanoscale Res Lett 6(1):427. doi: 10.1186/1556-276X-6-427.

Curling SF, Clausen CA, Winandy JE (2002) Relationships between mechanical properties, weight loss, and chemical composition of wood during incipient brown-rot decay. For Prod J 52(7/8):34–39.

DeGroot RC (1976) Wood Decay Ecosystem in Residential Construction, Forest Service, U.S. Department of Agriculture, Trees and Forests for Human Settlements, Proceedings, XVIth IUFRO World Congress, p. 334–352.

França TS, Stokes CE, Tang JD (2018) Durability of cross laminated timber against termite damage. In Proc, 61st International Convention of Society of Wood Science and Technology and Japan Wood Research Society. Soc Wood Sci Technol, Monona, WI. https://research.fs.usda.gov/treesearch/57651

França TSFA, Stokes CE, and Tang JD (2022) Development of a modified standard termite test for mass timber products. Wood Fiber Sci 54(1):24–34. https://doi.org/10.22382/wfs-2022-03

Freeman MH, McIntyre CR (2008) Copper-based wood preservatives. For Prod J 58(11):6–27. https://scispace.com/pdf/copper-based-wood-preservatives-a-comprehensive-review-of-2c43acf2l9.pdf

Garbacz M, Malec A, Duda-Saternus S, Suchorab Z, Guz L, Lagod G (2020) Methods for early detection of microbiological infestation of buildings based on gas sensor technologies. Chemosensors 8(1):7. https://doi.org/10.3390/chemosensors8010007

Hastrup ACS, Green III F, Clausen CA, Jensen B (2005) Tolerance of Serpula lacrymans to copper-based wood preservatives. Int Biodeter Biodegr 56(3):173–177. https://doi.org/10.1016/j.ibiod.2005.06.008

Khademibami L, Bobadilha GS (2022) Recent developments studies on wood protection research in academia: A review. Front For Glob Change 5:793177. https://doi.org/10.3389/ffgc.2022.793177

Kim GH, Hwang WJ, Yoshimura T, Imamura Y (2010) Laboratory evaluation of the termiticidal efficacy of copper HDO. J Wood Sci 56:166–168. https://doi.org/10.1007/s10086-009-1072-y

Kirker GT, Ohno KM, Arango R, Mankowski ME (2021) Overview and progress of FPL research into mass timber durability. In Proc 117th Annual Meeting of the American Wood Protection Association, July 27–29, 2021, Nashville, TN. AWPA, Birmingham, AL 117:133–138. https://research.fs.usda.gov/treesearch/63653

Lim H, Tripathi S, and Tang JD (2020) Bonding performance of adhesive systems for cross-laminated timber treated with micronized copper azole type C (MCA-C). Constr Build Mater 232:117208. https://doi.org/10.1016/j.conbuildmat.2019.117208

Lin LD, Chen YF, Wang SY, Tsai MJ (2009) Leachability, metal corrosion, and termite resistance of wood treated with copper-based preservative. Int Biodeter Biodegr 63(4):533–538. https://doi.org/10.1016/j.ibiod.2008.07.012

Loferski JR (1999) Technologies for wood preservation in historic preservation. Arch Mus Inform 13(3):273–290. https://doi.org/10.1023/A:1012468326445

Lorenz LF, Frihart C (2006) Adhesive bonding of wood treated with ACQ and copper azole preservatives. For Prod J 56(9):90.

Maeda K, Ohta M, Momohara I (2015) Relationship between the mass profile and the strength property profile of decayed wood. Wood Sci Tech 49:331–344. https://doi.org/10.1007/s00226-014-0696-2

Mankowski ME, Shelton TG, Kirker G, Morrell JJ (2022) Assessment of termite and decay damage to mass timber elements in AWPA ground proximity and above ground field tests in southern Mississippi. In Proc 118th Annual Meeting of the American Wood Protection Association, May 15–17, 2022, Charleston, SC. AWPA, Birmingham, AL 118:218–226. https://research.fs.usda.gov/treesearch/65037

Meyer L, Brischke C (2015) Fungal decay at different moisture levels of selected European-grown wood species. Int Biodeter Biodegr 103:23–29. https://doi.org/10.1016/j.ibiod.2015.04.009

Novotný Č, Cajthaml T, Svobodová K, Šušla M, Šašek V (2009) Irpex lacteus, a white-rot fungus with biotechnological potential. Folia Microbiol 54:375–390. https://doi.org/10.1007/s002530000432

Ohno KM, Bishell AB, Stanosz GR (2020) Gene expression analysis of three putative copper-transporting ATPases in copper-tolerant Fibroporia radiculosa. Front Microbiol 11:586940. https://doi.org/10.3389/fmicb.2020.586940

Ong Z, Mat Arip MN, Lipeh SL, Besserer A, Brosse N, Fredon E, Singham GV, Yuen KH, Lee HL (2025) Advances in wood preservation technology: A review of conventional and nanotechnology preservation approaches. BioRes 20(3):8209–8255. https://doi.org/10.15376/biores.20.3.Ong

Palanti S, Feci E, Predieri G, Francesca V (2012) Copper complexes grafted to amino-functionalized silica gel as wood preservatives against fungal decay: mini-blocks and standard test. BioRes 7(4):5611–5621. https://doi.org/10.1007/s00226-010-0396-5

Quin F (2023) Expanding the market of biomaterials. Dissertation, Mississippi State University, 30421646, 115 p. https://scholarsjunction.msstate.edu/td/5803

Quin F, Ayanleye S, França TSFA, Shmulsky R, Lim H (2024) Bonding Performance of preservative treated cross-laminated timber (CLT) posttreated with CU-based preservatives. For Prod J 73(4):326–338. 10.13073/FPJ-D-23-00031

Quin F, França TSFA, Undadi H, Shmulsky R, França FJN, Henfield B (2025) Bonding durability and rolling shear strength of commercially produced southern yellow pine cross-laminated timber Treated with micronized copper azole (MCA). BioRes 20(2):3773–3787. https://doi.org/10.15376/biores.20.2.3773-3787

Syahirah YA, Anwar UMK, Sh L, Ong CB, Asniza M, Paridah MT (2025) The properties of Cross Laminated Timber (CLT): A review. Int J Adhes Adhes 138(2025):103924. https://www.sciencedirect.com/science/article/pii/S0143749624003063

Schmidt, O (2006). Wood and tree fungi: biology, damage, protection, and use. Springer-Verlag Berlin, Heidelberg. https://doi.org/10.1007/3-540-32139-X

Schultz TP, Nicholas DD (2010) A proposed accelerated field stake test for rapid assessment of wood preservative systems. Holzforschung 64(5):673–679. https://doi.org/10.1515/hf.2010.071

Shelton TG, Grace JK (2003) Termite physiology in relation to wood degradation and termite control. In Wood Deterioration and Preservation, Goodell B, Nicholas DD, Schultz TP eds. Ch. 13, pp. 242–252. https://doi.org/10.1021/bk-2003-0845.ch013

Sienkiewicz N, Buultjens TEJ, White NA, Palfreyman JW (1997) Serpula lacrymans and the heat-shock response. Int Biodeter Biodegr 39(2-3):217–224. https://doi.org/10.1016/S0964-8305(97)00016-4

Tascioglu C, Goodell B, Lopez-Anido R (2003) Bond durability characterization of preservative treated wood and E-glass/phenolic composite interfaces. Compos Sci and Technol 63(7):979–991. https://doi.org/10.1016/S0266-3538(03)00013-7

Taylor A, Denavit M, Lloyd J, Kim G, Kirker G, Mankowski M, (2023) Borate treatment of CLT panels using vacuum: A proof of concept. For Prod J 73(1):24–30. https://doi.org/10.13073/FPJ-D-22-00060

VanAcker J, Li W, Jiang X, Durimel M, De Ligne L, Parakhonskiy B, Skirtach A, Van den Bulcke J (2023) Combining wood protection options to enhance resistance against decay and improve fire safety of engineered wood products like CLT. In 16th International Conference on Durability of Building Materials and Components (DBMC), Li K, Fang D eds, CIMNE, Beijing, China. https://doi.org/10.23967/c.dbmc.2023.008

Verma M, Sharma S, and Prasad R (2009) Biological alternatives for termite control: a review. Int Biodeter Biodegr 63(8):959–972. https://doi.org/10.1016/j.ibiod.2009.05.009

Walker JC, Archer K, Lebow S (2006) Wood preservation. Primary wood processing: principles and practice, pp. 297–338. https://doi.org/10.1007/1-4020-4393-7_9

Wang JY, Stirling R, Morris PI, Taylor A, Lloyd J, Kirker G, Lebow S, Mankowski ME (2018) Durability of mass timber structures: A review of the biological risks. Wood Fiber Sci 50:110–127. https://wfs.swst.org/index.php/wfs/article/view/2655

Watkinson SC, Eastwood DC (2012) Serpula lacrymans, wood and buildings. In Advances in Applied Microbiology 78:121–149). Academic Press. https://doi.org/10.1016/B978-0-12-394805-2.00005-1

Published

2026-07-20

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