Discrimination, Near InfraRed Spectroscopy, portable spectrometer, PLSDA, Dalbergia, Madagascar


This study focused on the use of Near InfraRed (NIR) Spectroscopy to address the lack of tools and skills for wood identification of Dalbergia species from Madagascar. Two sample sets of 41 wood blocks and 41 wood cores belonging to four Dalbergia species (D. abrahamii, D. chlorocarpa, D.greveana, and D. pervillei) were collected in the northern and western regions of Madagascar. Sapwood and heartwood NIR spectra were measured onwood at 12% moisture content by using a portable VIAVI MicroNIR 1700 spectrometer. Four discrimination models corresponding to sapwood and heartwood of the two sample forms were developed using Partial Least Square Discriminant Analysis (PLSDA).

Good accuracy of 83.3% and 81.8% were obtained from the heartwood-based PLSDA models respectively for wood blocks and wood cores samples. All D. chlorocarpa samples were well-classified by the two models. Results highlighted the potential of portable NIR Spectroscopy as a helpful tool tosupport sustain- able management and trade of Madagascar’s Dalbergia species. Further studies are, however, needed for its operational use in identification routine.

Author Biography

Andry Clarel Raobelina, School of Agronomy of the University of Antananarivo

I am a PHD student in the Department of Forestry and Environment, at the School of Agronomy of the University of Antananarivo.


Andriambololonera S (2013) Rapport de l’étude sur les taxons de bois précieux Diospyros spp. et Dalbergia spp. en vue de leur inscription dans l’Annexe II de la CITES. Tech report. 14pp

Berrar D (2018) Cross Validation. Encyclopedia of Bioinformatics and Computational Biology 1: 542-545.

Bertrand D (2005) Étalonnage multidimensionnel : application aux données spectrales. Techniques de l'Ingénieur. 264 pp.

Braga JWB, Pastore TCM, Coradin VTR, Camargos JAA, Silva ARD (2011) The use of Near infrared Spectroscopy to identify solid wood specimens of swietenia macrophylla (CITES appendix II). IAWA J 32(2):285–296.

CITES (2020) Interpretation and implementation matters general compliance and enforcement. Report of Madagascar on the implementation of decision 18.96. Twenty-fifth meeting of the Plants Committee. Geneva (Switzerland), 17 and 20-23 July 2020. 12 pp.

CITES (2019). Identification materials. (24 April 2021).

Costa EVS, Rocha MFV, Hein PRG, Amaral EA, dos Santos LM, Brandão LEVS, Trugilho PF (2018) Influence of spectral acquisition technique and wood anisotropy on the statistics of predictive near infrared-based models for wood density. J Near Infrared Spectrosc 26(2):106–116.

Environmental Investigation Agency (2016) EIA 2016. Lost years Fight against Traffick. Malagasy precious woods, an EIA Briefing for CITES SC67 and CoP17. (24 April 2021)

Eriksson L, Byrne T, Johansson E, et al (2013) Multi- and Megavariate Data Analysis Basic Principles and Applications, volume 1. Umetrics Academy, Sweden. 521 pp.

Evans PD, Mundo IA, Wiemann MC, Chavarria GD, McClure PJ, Voin D, Espinoza EO (2017) Identification of selected CITES-protected Araucariaceae using DART TOFMS. IAWA J 38(2):266–281.

Fatima T, Srivastava A, Somashekar PV, Hanur VS, Rao MS (2019) Development of DNA-based species identification and barcoding of three important timbers. Bull Natl Res Cent 43(1):1–17.

Freire CSR, Silvestre AJD, Neto CP (2005) Lipophilic extractives in Eucalyptus globulus kraft pulps. Behavior during ECF bleaching. J Wood Chem Technol 25(1-2):67–80.

Hajj F, Poszwa A, Bouchez J, Guérold F (2017) Radiogenic and “stable” strontium isotopes in provenance studies: A review and first results on archaeological wood from shipwrecks. J Archaeol Sci 86(2017):24–49.

Hassold S, Lowry PP, Bauert MR, Razafintsalama A, Ramamonjisoa L, Widmer A (2016) DNA barcoding of Malagasy rosewoods: Towards a molecular identification of CITES-Listed Dalbergia species. PLoS One 11(6):1-17.

Hein PRG, Lima JT, Chaix G (2010) Effects of sample preparation on NIR spectroscopic estimation of chemical pproperties of Eucalyptus urophylla S.T. Blake wood. Holzforschung 64(1):45-54.

Helcoski R, Tepley AJ, McGarvey JC, Gonzalez-Akre E, Meakem V, Thompson JR, Anderson-Teixeira KJ (2019) No significant increase in tree mortality following coring in a temperate hardwood forest. Tree-Ring Res 75(1):67–72.

Hillis WE (1971) Distribution, properties and formation of some wood extractives. Wood Sci Technol 5(4):272–289.

Kaner J, Jiufang L, Yongji X, Ioras F (2013) a reevaluation of woods used in chinese histroric furniture. Bulletin of the Transilvania University of Brasov 6(55):9-18.

Micha H, Michael J, Krehan H (2009) Control of origin of larch wood: discrimination between European (Austrian) and Siberian origin by stable isotope analysis. Rapid Commun Mass Spectrom 23(2009):3688–3692.

Morais MC, Pereira H (2012) Variation of extractives content in heartwood and sapwood of Eucalyptus globulus trees. Wood Sci Technol 46:(4)709–719.

Pastore TCM, Braga JWB, Coradin VTR, Magalhães WLE, Okino EYA, Camargos JAA, De Muñiz GIB, Bressan OA, Davrieux F, (2011) Near infrared spectroscopy (NIRS) as a potential tool for monitoring trade of similar woods: Discrimination of true mahogany, cedar, andiroba, and curupixá. Holzforschung 65(1):73–80.

Patel ER (2007) Looging af rare Rosewood and Palissandre (Dalbergia spp) within Marojejy National Park, Madagascar. Madag Conserv Dev 2(1):11-16.

Perez M, Marconi E (2018) Wooden Musical Instruments - Different Forms of Knowledge: Book of End of WoodMusICK COST Action P1302. Phillarmonie de Paris, France. 415 pp.

Randriamalala H, Liu Z (2010) Rosewood of Madagascar: Between democracy and conservation. Madagascar Conserv Dev 5(1):11–22.

Ratsimbazafy C, Newton DJ, Ringuet S, (2016) Timber island : rosewood and ebony trade of Madagascar. Traffic report. 125 pp.

Razafimahatratra AR, Belloncle C, Chaix G, Raobelina AC, Ramananantoandro T (2019) Variabilité des propriétés chimiques (extractibles et phénols totaux) de quelques espèces de bois précieux de Madagascar (Dalbergia spp. et Diospyros spp.). Pages 92-95 in Actes des 8e journées scientifiques du GDR 3544 Sciences du Bois. Epinal : LERMAB; ENSTIB. Journées du GDR 3544 "sciences du bois". 8, 18-20 November 2019, Epinal (France).

Rossard V, Boulet JC, Gogé F, Latrille E, Roger JM (2016) ChemFlow, chemometrics using Galaxy. Galaxy Community Conference - GCC2016. Indiana University. USA, Jun 2016, Bloomington, United States.

Routa, J., Brännström, H., Anttila, P., Mäkinen, M., Jänis, J., & Asikainen, A. (2017). Wood extractives of Finnish pine, spruce and birch – availability and optimal sources of compounds. 55 pp.

Saha S, Shilpi JA, Mondal H, Hossain F (2013) Ethnomedicinal , phytochemical , and pharmacological profile of the genus Dalbergia L . ( Fabaceae ). Phytophramacology 4(2):291–346.

Sandak A, Sandak J, Negri M (2011) Relationship between near-infrared (NIR) spectra and the geographical provenance of timber. Wood Sci Technol 45(1):35–48.

Schmitz, N. (ed.), H. Beeckman, C. Blanc-Jolivet, L. Boeschoten, J.W.B. Braga, J.A. Cabezas, G. Chaix, S. Crameri, B. Degen, V. Deklerck, E. Dormontt, E. Espinoza, P. Gasson, V. Haag, S. Helmling, M. Horacek, G. Koch, C. Lancaster, F. Lens, A. Lowe, S. Martínez-Jarquín, J.A. Nowakowska, A. Olbrich, K. Paredes-Villanueva, T.C.M. Pastore, T. Ramananantoandro, A.R. Razafimahatratra, P. Ravindran, G. Rees, L.F. Soares, N. Tysklind, M. Vlam, C. Watkinson, E. Wheeler, R. Winkler, A.C. Wiedenhoeft, V.Th. Zemke, P. Zuidema (2020). Overview of current practices in data analysis for wood identification. A guide for the different timber tracking methods. Global Timber Tracking Network, GTTN secretariat, European Forest Institute and Thünen Institute. 141 pp.

Schuurman D, Lowry II P (2009) The Madagascar rosewood massacre. Madagascar Conserv Dev 4(2):98–102.

Shou G, Zhang W, Zhao D (2014) Application of near infrared spectroscopy for discrimination of similar rare woods in the Chinese market. J Near Infrared Spectrosc 22:423–432.

Snel FA, Braga JWB, da Silva D, Wiedenhoeft AC, Costa A, Soares R, Coradin VTR, Pastore TCM, (2018) Potential field-deployable NIRS identification of seven Dalbergia species listed by CITES. Wood Sci Technol 52(5):1411-1427.

Tsuchikawa S, Kobori H (2015) A review of recent application of near infrared spectroscopy to wood science and technology. J Wood Sci 61(3):213–220.

Van Mantgem PJ, Stephenson NL (2004) Does coring contribute to tree mortality? Can J For Res 34(11):2394–2398.

Wang SN, Zhang F Da, Huang AM, Zhou Q (2016) Distinction of four Dalbergia species by FTIR, 2nd derivative IR, and 2D-IR spectroscopy of their ethanol-benzene extractives. Holzforschung 70(6):503–510.

Wegst UGK (2006) Wood for sound. Am J Bot 93(10):1439–1448.

Wold S, Sjöström M, Eriksson L (2001) PLS-regression: A basic tool of chemometrics. Chemom Intell Lab Syst 58(2):109–130.

Yan H, Siesler HW (2018) Hand-held near-infrared spectrometers: State-of-the-art instrumentation and practical applications. NIR news 29(7):8–12.

Yin X, Huang A, Zhang S, Liu R, Ma F (2018) Identification of three Dalbergia species based on differences in extractive components. Molecules 23(9):1–11.

Zhang M, Liu Y, Yang Z (2015) Correlation of Near Infrared Spectroscopy Measurements with the Surface Roughness of Wood. BioResources 10(4):6953–6960.

Zhang M, Zhao G, Guo J, Wiedenhoeft AC, Liu CC, Yin Y (2019) Timber species identification from chemical fingerprints using direct analysis in real time (DART) coupled to Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS): Comparison of wood samples subjected to different treatments. Holzforschung 73(11):975–985.






Research Contributions