• Charles Warren Edmunds North Carolina State University
  • Perry Peralta North Carolina State University
  • Ilona Peszlen
  • Ratna Sharma-Shivappa North Carolina State University
  • Richard Giles Universidad Interamericana de Peurto Rico
  • Stephen Kelley North Carolina State University
  • Vincent Chiang North Carolina State University


Ceriporiopsis subvermispora, wood degradation, white-rot fungi, fungal degradation, sweetgum


Fungal pretreatment has been explored as a low-cost and an environmentally friendly method to increase the reactivity of lignocellulosic biomass prior to further processing for pulp and paper, biofuels, and biochemicals.  Testing of genetically modified wood specimens is becoming increasing popular due to advances in the field of biomass research and the potential to greatly enhance the wood’s chemical and physical properties.  However, experimental methods for small juvenile wood specimens is not well characterized.  This research utilized sweetgum (Liquidambar styraciflua) to examine several variables in the inoculation and incubation procedure of small wood specimens degraded by white-rot fungus, Ceriporiopsis subvermispora, to find which method results in a sufficient amount of biomass degradation and low variation between replicates.  The variables examined include inoculation medium, wood particle size, and incubation container.  Increased fungal growth, weight loss values, and significant reduction in variation of weight loss were observed when using the malt extract fungal culture to directly inoculate the wood samples and closing with loose caps, instead of filtering, rinsing, and suspending the mycelium and sealing with Parafilm.

Author Biographies

Charles Warren Edmunds, North Carolina State University

Ph.D. Candidate

Department of Forest Biomaterials

Department of Agricultural and Biological Engineering

North Carolina State University

Campus Box 8005, Raleigh, NC 27695


Perry Peralta, North Carolina State University

Associate Professor

Department of Forest Biomaterials

North Carlina State University

Campus Box 8005, Raleigh, NC 27695


Ilona Peszlen

Associate Professor

Department of Forest Biomaterials

North Carlina State University

Campus Box 8005, Raleigh, NC 27695

Ratna Sharma-Shivappa, North Carolina State University

Associate Professor

Department of Biological and Agricultural Engineering

North Carolina State University

Campus box 7625, Raleigh, NC 27695

Richard Giles, Universidad Interamericana de Peurto Rico

Adjunct Professor

Departmento de Ciencias Y Tecnologia 

Universidad Interamericana de Puerto Rico

P.O. Box 4050, Arecibo, Puerto Rico, USA 00614

Stephen Kelley, North Carolina State University

Department Head and Professor

Department of Forest Biomaterials

North Caronlina State University

Campus Box 8005, Raleigh, NC 27695

Vincent Chiang, North Carolina State University


Department of Forest Biomaterials

North Carolina State University

Campus Box 8005, Raleigh, NC 27695


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