Thickness Variation in Ultramicrotomed Wood Sections
Keywords:
Ultrathin sections, cell-wall layers, ultramicrotome, section thickness, secondary wall, electron microscopy, ultrastructure, anatomyAbstract
Electron micrographs of ultrathin sections reveal variation in the section thickness of the various cell-wall layers. The variation was present in cell walls that were not parallel to the cutting edge of the knife and absent from cell walls that were parallel to the knife edge. Although only a speculative explanation for this cutting artifact is offered, its presence in the various cell types is clearly illustrated.References
Asunmaa, S., and B. Steenberg. 1957. Relative scattering densities of pine heartwood, pine sapwood and spruce. Svensk Papperstidn. 60:751-761.nCöté, W. A., and A. C. Day. 1969. Wood ultrastructure of the southern yellow pines. Tech. Publication No. 95. State University of New York, College of Environmental Science and Forestry. Syracuse. New York.nFergus, B. J., A. R. Procter. S. A. N. Scott, and D. A. I. Goring. 1969. The distribution of lignin in the spruce wood as determined by ultraviolet microscopy. Wood Sci. Technol. 3:117-138.nPreusser, H. J., H. H. Dietrichs, and H. Gottwald. 1961. Elektronenmikroskopische Untersuchungen an Ultradünnschitten des Markstrahlparenchyms der Rotbuche. Holzforschung 15:65-75. Translated by M. Schroeder.n
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