Thickness Variation in Ultramicrotomed Wood Sections
Keywords:Ultrathin sections, cell-wall layers, ultramicrotome, section thickness, secondary wall, electron microscopy, ultrastructure, anatomy
AbstractElectron micrographs of ultrathin sections reveal variation in the section thickness of the various cell-wall layers. The variation was present in cell walls that were not parallel to the cutting edge of the knife and absent from cell walls that were parallel to the knife edge. Although only a speculative explanation for this cutting artifact is offered, its presence in the various cell types is clearly illustrated.
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