Polysaccharide-Degrading Complex Produced in Wood and in Liquid Media by the Brown-Rot Fungus <i>Poria Placenta</i>


  • Terry L. Highley
  • Karl E. Wolter
  • Faye J. Evans


Hemicellulase, glycosidase, decay, degradation, enzyme purification


The polysaccharide-degrading enzymes produced by Poria placenta in decayed wood and liquid media were compared qualitatively and quantitatively. A single carbohydrate-degrading complex was isolated and purified from wood and liquid cultures that was active on both polysaccharides and glycosides. Quantitative differences in enzyme activities from decayed wood versus liquid media were observed. However, the purified extracellular carbohydrate-degrading complex isolated from decayed wood and from liquid cultures must be structurally similar because of similar isoelectric points, electrophoretic properties, and molecular sieving properties.


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