The Effect of Polyphenol Extraction on Enzyme Degradation of Bordered Pit Tori

Authors

  • Richard J. Thomas

Keywords:

Bordered pit membrane, torus, pectinase, cellulase, lignin, polyphenols, polyphenol extraction, chemical composition, ultrastructure

Abstract

Extraction of polyphenols from the heartwood of loblolly pine, Fraser fir, and red spruce, followed by pectinase and cellulase treatments, resulted in the removal of chemical constituents and the creation of openings in the torus of bordered-pit membranes. The results suggest that during heartwood formation polyphenols rather than lignin are produced and deposited within the torus.

References

Bamber, R. K. 1961. Staining reaction of the pit membrane of wood cells. Nature 191:409-410.nBauch, J., W. Liese, and F. Scholz. 1968. Über die Entwicklung und stoffliche Zusammen-setzung der Hoftupfel membranen von Lang-stracheiden in Coniferen. Holzforschung 22(5):144-153.nBauch, J., and H. Berndt. 1973. Variability of the chemical composition of pit membranes in bordered pits of gymnosperms. Wood Sci. Technol. 7:6-19.nConn, E. E. 1964. Enzymology of phenolic biosynthesis. J. B. Harbone, ed. In Biochemistry of phenolic compounds. Academic Press, N. Y.nFirenzuoli, A. M., P. Vanni, and E. Mastronuzzi. 1969. The effect of some aromatic compounds on pure enzymes and their subsequent reactivation by PVP and Tween 80. Phytochemistry 8(1):61-64.nFrey-Wyssling, A., K. Mühlethaler, and H. Moor. 1956. Elektronen-mikroskopische Prapartionsartefakte dünner Cellulosemembranen. Mikroskopie 11:219-224.nNicholas, D. D., and R. J. Thomas. 1968. The influence of enzymes on the structure and permeability of loblolly pine. Proc. Am. Wood-Pres. Assoc. 64:70-76.nRydholm, S. 1964. Pulping processes. Inter-science Publishers, New York, N. Y. P. 886.nSarkanen, K. V., and C. H. Ludwig. 1971. Definition and nomenclature. In Sarkanen and Ludwig, eds. Lignins: occurrence, formation, structure and reactions. Wiley-Inter-science, N. Y.nSabkanen, K. V., and H. L. Hergert. 1971. Classification and distribution. Pages 81-89. In Sarkanen and Ludwig, eds., Lignins: Occurrence, formation, structure and reactions. Wiley-Interscience, N. Y.nThimann, K. V. 1964. Formation of wood in forest trees. Edited by M. Zimmerman. Academic Press, N. Y. P. 452.nWardrop, A. B. 1971. Occurrence and formation in plants. In Sarkanen and Ludwig, eds., Lignins: Occurrence, formation, structure and reactions. Wiley-Interscience.n

Downloads

Published

2007-06-05

Issue

Section

Research Contributions